The Class 12 CBSE Biotechnology exam is scheduled for March 21, 2025.
New Delhi: The Central Board of Secondary Education (CBSE) is conducting the Class 12 board exams for the 2025 academic year, with the Biotechnology exam scheduled for March 21, 2025. Students can visit the official CBSE website to access sample papers and prepare effectively.
Important Questions for CBSE Class 12 Biotechnology Exam 2025
- Who invented the dideoxynucleotide chain termination method? Explain the method schematically.
- Why is the dye termination method considered better than the chain termination technique? Mention any two reasons.
- Why is the autoradiogram read from bottom to top (anode to cathode)?
- Why do bacteria produce restriction enzymes? How do they protect their own DNA from its action?
- Write the basic steps (starting from an explant) involved in obtaining a regenerated plant. Suggest any two applications of plant tissue culture.
- How can intergeneric somatic hybrids be made? Provide an example. What are cybrids?
- How are edible vaccines advantageous over recombinant vaccines produced by bacterial fermentation?
- A protein with a molecular weight of 10,000 contains charges of 4, 3, 2, and 1. Calculate the m/z ratio at which a mass spectrometer would detect these protein ions. Also, draw the corresponding Mass Spectrum.
- A doctor has to prescribe a protein-rich diet to sportsmen to improve performance. What three parameters should the doctor consider while selecting protein sources?
- Selection is a crucial step in genetic engineering. Given ampicillin and tetracycline antibiotics, which selection technique could be used to differentiate between recombinant and non-recombinant cells?
- Which microbial cells have been extensively used for functional expression of eukaryotic genes, and why?
- Illustrate the essential steps involved in raising a chimeric mouse using stem cell technology.
- A eukaryotic polypeptide hormone gene was isolated, cloned, and over-expressed in a bacterium. However, the purified polypeptide failed to function in the original organism. Suggest possible reasons for the recombinant protein’s inactivity.
Students are advised to revise key concepts, practice sample papers, and focus on application-based questions for better performance in the exam.
